Department of Chemical and Bioresource Engineering, Colorado State University, Fort Collins, CO, 80523, 303-491-5252
Growth of a Pseudomonas fluorescens species has been demonstrated with TNT as the sole nitrogen source and acetate as the carbon source. While only 10% of the transformed TNT was accounted for by HPLC analysis, growth measurements indicate that a significant amount of the TNT nitrogen present was converted to biomass. 2-amino-4,6-dinitrotoluene was identified as a dead end metabolite. Picric acid and trinitrobenzene were also identified as intermediates and may be part of the nitrogen use pathway. The use of TNT as a nitrogen source may lead to ring cleavage and mineralization, since growth has also been observed with TNT as the sole nitrogen and carbon source
This paper is from the Proceedings of the 10th Annual Conference on Hazardous Waste Research 1995, published in hard copy and on the Web by the Great Plains/Rocky Mountain Hazardous Substance Research Center.