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P31DEGRADATION OF TNT AND DERIVATIVES BY A BACTERIAL CONSORTIUM |
G.K. Vasilyeva(1), R.A. Drijber(1), B.-T. Oh(1), P.J. Shea(1), S.D. Comfort(1), and J.-M. Bollag(2), (1)Department of Agronomy, University of Nebraska, Lincoln, NE, 68583-0915, (2)Center for Bioremediation and Detoxification, The Pennsylvania State University, University Park, PA, 16802-4900 |
A bacterial consortium aerobically degraded 2,4,6-trinitrotoluene (TNT) in the presence of cosubstrates (sugars and simple organic acids). Within 16 h, TNT (100 mg L-1) disappeared and nitrite (approximately 4% of total nitrogen) was detected in the culture medium. Small amounts of monoaminodinitro reduction products of TNT (2-amino-4,6-dinitrotoluene and 4-amino-2,6-dinitrotoluene) were also detected. In experiments with 14C-TNT, about one-half of the 14C remained in solution after centrifugation, mainly as unidentified polar and water-soluble products. 14CO2 production was limited to less than 0.8% of the l4C-TNT.
The remaining 14C was found in the precipitated fraction; only 10.4% of this 14C was extractable with methylene chloride and no TNT was detected. Results indicate that TNT was reduced to hydroxylaminodinitrotoluenes that were subsequently transformed to products contained in the precipitate. In separate experiments, the monoaminodinitrotoluene products of TNT metabolism were transformed at a slower rate than TNT, primarily to diaminonitrotoluenes that were persistent in the medium. The consortium also degraded 1,3,5-trinitrobenzene, while the dinitrotoluenes were only minimally degraded. Key words: TNT, munitions, bioremediation, transformation
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