Program Two
Thursday, May 22, 1997

Bioremediation Kansa B



T.M. Roane and I.L. Pepper, 429 Shantz Building #38, Soil, Water and Environmental Science, The University of Arizona, Tucson, AZ 85721 One-third of organically-polluted sites are also contaminated with metals; however, the bioremediation potential of such sites is not clear. While metals are thought to inhibit the abilities of microbial communities to degrade organic pollutants, several microbial-metal resistance mechanisms are known to exist. This study utilizes cadmium- resistant soil microorganisms to enhance the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) in the presence of cadmium.

Cadmium-resistant microorganisms were isolated from both a 40-year-old metal-contaminated soil and an uncontaminated soil. Interestingly, using BIOLOG and ERIC PCR fingerprints, it was found that the uncontaminated soils had a greater diversity of cadmium-resistant isolates at low concentrations of cadmium, while cadmium-contaminated soils had more diversity at higher cadmium concentrations. Preliminary identification of cadmium-resistant isolates included Bacillus, Pseudomonas, and Xanthomonas spp.

Isolates were resistant up to 80 ppm cadmium. Using conventional degradation studies, in conjunction with molecular analyses, the potential of these cadmium-resistant micro-organisms to degrade 2,4-D in the presence of cadmium was evaluated.

Key words: metal-resistant, microorganisms, co-contaminated soils, remediation

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Tuesday, May 20, 1997

Metals Kansa A

Remediation of Munitions Compounds Kansa B

Analytical Methods Kansa C/D

General Topics Kansa B

Wednesday, May 21, 1997

Metals Kansa A

Zero-Valent Metals Kansa A

Remediation Kansa A

Vegetation-based Remediation Kansa B

Partnerships & Innovative Technologies Kansa C/D

Nonaqueous Phase Liquids Kansa C/D

Thursday, May 22, 1997

Biofilms & Barriers Kansa A

Bioremediation Kansa B

Partnerships & Technology Innovations Kansa C/D

Remediation Kansa C/D


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