71

DIRECT OBSERVATION OF A BIOFILM IN SOIL USING FOURIER TRANSFORM INFRARED SPECTROSCOPY

D.A. Buttry, S. J. Clay, J.P. Turner, and L.A. Bulla, Department of Chemistry, Department of Civil Engineering, and Department of Molecular Biology,University of Wyoming, Laramie, WY 82070

Biofilms produced from bacteria and exo-polysaccharides (EPS) are being investigated as a potential barrier to hazardous materials. With this biofilm, an initially porous soil matrix can become very impermeable, effectively producing a barrier. In this study, fourier transform infrared spectroscopy (FTIR) was used to monitor, in situ, the growth rate of a Beijerinckia indica biofilm in soil at the surface of a germanium internal reflection element (IRE). Production of the biofilm was monitored by observation of absorption bands attributed to exopolysaccharides and proteins.

Key words: biofilm, exopolysaccharides, bacterial adhesion

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Tuesday, May 20, 1997

Metals Kansa A

Remediation of Munitions Compounds Kansa B

Analytical Methods Kansa C/D

General Topics Kansa B

Wednesday, May 21, 1997

Metals Kansa A

Zero-Valent Metals Kansa A

Remediation Kansa A

Vegetation-based Remediation Kansa B

Partnerships & Innovative Technologies Kansa C/D

Nonaqueous Phase Liquids Kansa C/D

Thursday, May 22, 1997

Biofilms & Barriers Kansa A

Bioremediation Kansa B

Partnerships & Technology Innovations Kansa C/D

Remediation Kansa C/D

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